Part:BBa_K2802001:Design

PSP3 pag - PF promoter - PSP3 pag - RBS + RFP + d.Ter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 400
  Illegal AgeI site found at 1300
  Illegal AgeI site found at 1412
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

BBa_K2802000 and BBa_K518012 were joined together using 3A Assembly.

The 718 bp BBa_K2802000 (lane 2) and 828 bp BBa_K518012 parts were joined together to make 1554 bp BBa_K2802001 (lane 3). Parts were digested with EcoRI and PstI.

Source

BBa_K2802000 + BBa_K518012